Mass Spectrometry and Synthesizing / Sequencing Facility Service Fees
Off-campus users will be charged at 3x of Princeton
University users
Mass Spectrometry
| LTQ OrbiTrap |
$ 30 / hour |
| LCQ |
$ 12 / hour |
| Triple Quad |
$ 12 / hour |
| Startup Fee |
$ 75 / order |
| C18 nano column |
$ 50 / order |
| C18-SCX-C18 nano column |
$ 150 / order |
| Trypsin digestion |
$ 10 / digestion |
| Phosphopeptide Enrichment |
$ 50 / sample |
| Data analysis |
$ 75.00 / hour |
| Special experimental setup and design |
$ 75.00 / hour |
( add the cost of enzyme for other endopeptidases such as AspN, GluC. )
DNA Synthesis
| Scale |
25nmole |
100nmole |
0.2 umole |
1.0 umole |
ddRNAi |
Ultramer |
| Per base |
$0.2 |
$0.4 |
$0.64 |
$1.15 |
0.30 |
0.75 |
| Purification |
N/A |
$30 |
$45 |
$90 |
$ 95 |
$95 |
| IE-HPLC |
N/A |
$40 |
$60 |
N/A |
|
|
| Yield (min. OD) |
|
|
|
|
|
|
| Crude |
2 |
6 |
15 |
40 |
3nmole |
3nmole |
| Pure |
N/A |
1 |
4 |
20 |
|
|
| Plate price |
$0.12 |
$0.15 |
$0.35 |
$0.75 |
|
|
(Pricing for “Sameday” order and other modification can be found at IDTDNA.com.)
Peptide Synthesis
| Scale |
0.25 mmole |
|
|
| Setup fee |
100.00 |
|
|
| Per amino acid |
35.00 |
|
|
| Purification |
250.00 |
|
|
| Alpha Factor Peptide |
350.00 / 50mg |
|
|
| Yield (min.) : crude |
75-100 mg |
Purified |
30-50 mg |
N-terminal Protein Sequencing (Edman chemistry)
| First 5 amino acids |
120 / sample |
| Additional cycle |
$ 24 / cycle |
| Sample amount required |
10 pmole ( blotted on PVDF or in liquid ) |
( A minimum of $120 per sample will be charged. )
DNA Sequencing (Fee for other additional services can be found at www.GENEWIZ.com)
| Premix regular sample |
= $ 6.50 |
| 96well plate ( 48 min.) |
= $ 5.50 |
| 96well plate ( 94 min.) |
= $ 4.40 |
|
|
| Difficult samples |
= add $ 3.85 |
|
|
|
|
|
|
| PCR purification |
= add $ 2.20 |
| Amplification of DNA from Bacteria |
= $ 4.40 / sample |
Amplification of DNA from Bacteria 48 or more
|
= $ 2.20 / sample |
|
Fees 
