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Summary of Mass Spectrometry and Synthesizing / Sequencing Facility services Mass Spectrometry LTQ Orbitrap Hybrid mass spectrometer (from Thermo) interfaced with Eksigent nano LC To Order: Complete order form email to This e-mail address is being protected from spam bots, you need JavaScript enabled to view it and place the sample in the refrigerator in LTL 027/Synseq. DNA Synthesis and Purification Most oligonucleotide synthesis is subcontracted to IDT. Particularly long oligonucleotides, those containing special bases and/or those requiring HPLC purification will be synthesized in-house using a Model 392 Applied Biosystems DNA synthesizer. To order oligos: From IDT: please go to http://www.idtdna.com/Princeton-Molbio-Synseq set up an account, and order what you need from IDT. In-house: Complete order form and email to This e-mail address is being protected from spam bots, you need JavaScript enabled to view it To pickup: Oligo packages can be picked up in front of LTL 136. DNA Sequencing DNA sequencing is subcontracted to GENEWIZ and Macrogen companies. Genewiz: go to http://clims.genewiz.com/NewAccount.aspx, setup an account, complete an on-line order form, and place your samples together with a copy of the form in the drop off refrigerator in front of LTL-136. Data can be retrieved from the Genewiz server. For more info, please read the Genewiz-Princeton handout. Macrogen: go to http://orders.macrogenusa.com, setup an account, complete an on-line order form, and place your samples together with a copy of the form in the drop off refrigerator in front of LTL-136. Data can be retrieved from the Macrogen server. For more info, please go to http://macrogenusa.com Peptide Synthesis and Purification Peptide synthesis is performed on an Applied Biosystems Model 433A peptide synthesizer using FMOC solid phase synthesis (Merrifield) chemistry. The purification is performed using an Agilent 1100 reverse phase HPLC system. To Order: Complete order form and email to This e-mail address is being protected from spam bots, you need JavaScript enabled to view it .
N-terminal protein sequencing N-terminal protein sequencing using Edman degradation chemistry is subcontracted to another core facility. To Order: Complete order form email to This e-mail address is being protected from spam bots, you need JavaScript enabled to view it and place the sample in the refrigerator in LTL 027/Synseq. Real-Time PCR This machine has been moved to Lewis-Sigler Institute for Integrative Genomics. Please contact Donna Storton, This e-mail address is being protected from spam bots, you need JavaScript enabled to view it 8-7842 |