v-FLIP MC159 X-ray diffraction from data set at
X29 beam line at NSLS-I
Diffraction image for tetragonal lysozyme using
the RAXIS-IV++ detector and RuH3R home source
For an example of what data collection on a well-diffracting crystal looks like, here's a video of a 90° data collection (100 sec/0.5°) crammed into 7 seconds from our in-house source. Proteinase K is a strong diffractor and this data extends well past the edge of the detector at 1.6 Å resolution.
For straightforward cases (strong diffractors, available homologous structures) we can determine structures using data collected in-house. For other cases (novel structures or weak diffractors) we must use the ultra-bright tunable X-ray sources at synchrotrons. Our oft-used favorite is beam line X29 at Brookhaven National Lab which offers a bright beam that is capable of accessing the wavelengths most frequently used in structure determination of novel structures. NSLS-I ceases operations in fall 2014 to facilitate the operations of NSLS-II and in the intervening time between viable NSLS-I and NSLS-II beam lines we will be using synchrotrons at Cornell (CHESS) and Argonne (APS), amongst others.
For the in-house source we support the HKL3000 package for data collection and processing.XDS, MOSFLM, autoProc and Xia2 processing programs for data collected either in-house or at synchrotrons.