The Flow Cytometry Resource Facility provides support for faculty and student research at Princeton University, as well as for investigators outside the university at other academic institutes or private companies. In addition to providing access to the flow cytometers and cell sorting services, we provide technical support to assist in experimental design, data analysis and interpretation as well as development of novel flow cytometric based techniques.
Flow cytometry is a laser based analytical tool in which cells or small organisms in suspension flow at high speed past one or more focused laser light sources. Fluorescently labeled probes and dyes can be added to the sample to determine specific physical and/or biochemical properties of the cells. Optical filters and detectors direct and measure the scattered laser light as well as the fluorescence that is emitted from the cell. Various software platforms are used to acquire and analyze the data, which can be exported in several formats for presentation.
The facility analyzes and sorts a wide range of cell types and species including human, murine, porcine and insect cell lines as well as primary murine leukocytes and stem cells, bacteria, yeast, and C. elegans, which reflects the diversity of the research in the department.