FLUORESCENT DYES FOR CONFOCAL MICROSCOPY

BEST DYES FOR THE ZEISS LSM 510

* Dyes listed in order of preference and spectral performance
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 Laser Line Excitation
Dyes
Emission Color

 364 nm. UV
  • Hoechst 33342, for DNA 
  • Dapi, for DNA 
  • AlexaFluor-350 
  • Cascade Blue
  • AMCA
    •
  •  Violet / Blue 
    •

     457 nm Argon
  •  Lucifer Yellow 
    •
  •  Green / Yellow 
    •
     488 nm. Argon
  • AlexaFluor-488 
  • Oregon Green 
  • YOYO-1, for DNA 
  • Cy-2 
  • FITC 
  • PE 
    •
  • Green 
  • Green 
  • Green 
  • Green 
  • Green 
  • Red 
    •

    543 nm. HeNe
  • AlexaFluor-546 
  • AlexaFlour-568 
  • Tetramethyl Rhodamine
  • Lisamine Rhodamine
  • Propidium Iodide, for DNA 
  • BOBO-1 for DNA 
    •
  • Red 
    •

    633 nm. HeNe
  •  Cy-5 
  • AlexaFluor-633
  • TOTO-3, for DNA 
  • APC 
    •
  •  Deep Red 
    •
     
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