Contact: Joe Goodhouse at goodj@molecular.princeton.edu
Laser scanning confocal microscopy provides our researchers the ability to optically section whole specimens of cells and small organisms, such as developing fly and fish embryos, or very thick sections from brain and other organ tissues, that have been tagged with fluorescent or reflective probes. This technology provides us with the highest light microscope resolution obtainable, giving the scientist a clearer picture of sub cellular structures, function, and cellular or organism architecture. We can perform this on single or multiply labeled specimens. Our laboratory has more than 10 years experience of confocal imaging in mammalian cells, yeast cells, Drosophila and Zebra fish embryos and ovaries, bacteria, sections of brain and other tissues, in both fixed and live specimens. The laboratory has several software package for image analysis, processing and 3D image reconstruction of these confocal images.
The Zeiss LSM 510 Confocal System is a state of the art confocal, installed in October 1997, it has been upgraded several times with newer scan head and additonal filter sets for the simultaneous imaging of CFP and YFP. ThE 510 has 4 lasers producing 6 lines for fluorochrome excitation, 4 color detectors for fluorescent or reflective confocal imaging, and a transmitted light detector.
This confocal microscope was installed in 1991
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